A reliable method for storage of tailed phages.

نویسندگان

  • Piotr Golec
  • Kamil Dąbrowski
  • Monika S Hejnowicz
  • Agnieszka Gozdek
  • Joanna M Loś
  • Grzegorz Węgrzyn
  • Małgorzata B Lobocka
  • Marcin Loś
چکیده

A universal and effective method for long-term storage of bacteriophages has not yet been described. We show that randomly selected tailed phages could be stored inside the infected cells at -80°C without a major loss of phage and host viability. Our results suggest the suitability of this method as a standard for phage preservation.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

پیشرفتهای جدید در استفاده از باکتریوفاژها در درمان عفونتهای باکتریایی

Introduction: Resistance increase to a variety of antibiotics has caused microbiology researchers to develop new ways instead of the conventional treatment methods for preventing bacterial infections. Nowadays, Phages or bacterial contaminant particles are using as an efficient method in the treatment of many infectious diseases. The uses of live bacteriophages in the treatment of fatal disease...

متن کامل

Bacteriophages of Erwinia amylovora.

Fifty bacteriophage isolates of Erwinia amylovora, the causal agent of fire blight, were collected from sites in and around the Niagara region of southern Ontario and the Royal Botanical Gardens, Hamilton, Ontario. Forty-two phages survived the isolation, purification, and storage processes. The majority of the phages in the collection were isolated from the soil surrounding trees exhibiting fi...

متن کامل

Jumbo Bacteriophages: An Overview

Tailed bacteriophages with genomes larger than 200 kbp are classified as Jumbo phages, and are rarely isolated by conventional methods. These phages are designated "jumbo" owing to their most notable features of a large phage virion and large genome size. However, in addition to these, jumbo phages also exhibit several novel characteristics that have not been observed for phages with smaller ge...

متن کامل

Culture and molecular characterization of phages isolated from rainbow trout farms and sewage treatment plants and investigation of their effects on Yersinia ruckeri

In the present study bacteriophages isolated from rainbow trout farms and sewage treatment plants were genetically identified and their effectiveness on Yersinia ruckeri isolates from clinical cases of red mouth disease was investigated.  Fish samples suspected to red mouth disease were collected from rainbow trout farms located in west Azerbaijan. Y. ruckeri, the causative agent of red mouth d...

متن کامل

The first phage electron micrographs

The first phage electron micrographs were published in 1940 in Germany and proved the particulate nature of bacteriophages. Phages and infected bacteria were first examined raw and unstained. US American scientists introduced shadowing and freeze-drying. Phages appeared to be tailed and morphologically heterogeneous. Phage types identified by early electron microscopy include enterobacteriophag...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of microbiological methods

دوره 84 3  شماره 

صفحات  -

تاریخ انتشار 2011